It runs for 101 miles (163 km) through east central and eastern Nebraska.

They overlap for 5 miles (8.0 km), then NE 32 turns east near Newman Grove.

It turns east, meets Nebraska Highway 121, then enters Madison.

At the east end of Madison, NE 32 meets U.S. Highway 81.

After separating from US 275, NE 32 turns east again and meets U.S. Highway 77 near Oakland.