New York City agar

[1] The agar base is composed of:[1] Final pH ( at 25°C) 7.4±0.2 NYC Agar Base was originally developed by Fauer, Weisburd and Wilson[1][2][3] at the New York City Department of Health for selective isolation of pathogenic Neisseria species from clinical specimens.

[6] Proteose peptone, horse plasma, haemoglobin provide nutrients for the growth of N. gonorrhoeae and N. meningitidis.

The selective supplement added contains the antibiotics vancomycin, colistin, nystatin and trimethoprim, to suppress the accompanying flora.

The yeast autolysate supplement fulfils the CO2 requirements needed to enhance Neisseria growth.

[9] Also, presence of yeast autolysate reduces the lag phase of growth of Neisseria, thus enhancing both size and number of colonies.