The route runs 16.58 miles (26.68 km) between US 50 in Cleves to intersections with Central Avenue in Downtown Cincinnati.

The route runs through southern Hamilton County and serves western Cincinnati suburbs.

It acts as an alternate route to US 50 which runs along the Ohio River serving other villages including North Bend and Addyston, as well as the western neighborhoods of Cincinnati (Sayler Park and Riverside).

After bypassing the village of Cheviot, the route enters the city limits of Cincinnati, specifically the Westwood neighborhood.

SR 264 turns right at the bottom of the hill onto State Avenue heading south towards the Ohio River.