Osthofen (German: [ˈɔstˌhoːfn̩] ⓘ) is a town in the middle of the Wonnegau in the Alzey-Worms district in Rhineland-Palatinate, Germany.

Archaeological finds have established that the Osthofen municipal area was already settled at least four thousand years ago.

It is believed that Osthofen was founded by people from either the now amalgamated village of Mühlheim or the Merovingian royal palace that once stood in Worms-Neuhausen.

The municipal election held on 7 June 2009 yielded the following results:[3] The town's arms might be described thus: Sable a lion rampant Or armed, langued and crowned gules, issuant from dexter chief the sun and in an arc from dexter to middle base three mullets, all of the second.

The town's earliest seals come from the 14th century, but they show a crane under a cross, a composition of unknown meaning.

The sun and stars (or heraldically, mullets) are canting charges, as they are meant to suggest the direction “east”, which is the first part of the town's name, the German word being Ost(en).