Our Lady of Guadalupe Seminary of the Priestly Fraternity of St. Peter, a Society of Apostolic Life in the Catholic Church, is located in Denton, Nebraska.

The seminary was canonically established in 1988 by Pope John Paul II in accordance with his letter Ecclesia Dei adflicta and opened in 2000.

Established in Denton, Nebraska, near Lincoln, it was founded primarily to serve English-speaking seminarians for the Fraternity from North America and to provide traditionalist Catholic education.

The seminary has also housed members of the Knights of the Holy Eucharist, brothers of the Franciscan tradition who originate from the Shrine of the Most Blessed Sacrament.

The seminarians receive training in the ancient Tridentine liturgy as it existed in 1962, prior to and during the Second Vatican Council; Gregorian chant is also an integral part of the education.