Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors.

PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.

PC substrate is polyunsaturated and resides in the membrane separately from lipid rafts near phosphatidylinositol 4,5-bisphosphate (PIP2).

When PIP2 levels increase, PLD2 trafficks to PIP2 where it encounters its substrate PC.

Scaffolding proteins that interact with PLD2 likely changes its preference of lipid rafts vs PIP2.