Padbury Brook is a stream/river in Oxfordshire and Buckinghamshire; it is a small tributary of the River Great Ouse approximately 12 km in length.

[2][better source needed] The brook heads eastward under the A4421 south of Newton Morrell in Oxfordshire, onwards north of Godington, then on into Buckinghamshire.

Padbury Brook then flows north-east in a wide and shallow valley passing north-east of Padbury, the brook goes under the A413 road, then under the A421 road (Buckingham to Milton Keynes road), then under Thornborough Bridge and on to King's Bridge, where it is then joined by its other arm, from the west (Thornborough) and becomes 'The Twins', before joining the River Great Ouse, east of Buckingham and west of Thornborough.

[2] Padbury Brook is home to fish such as the stone loach (Barbatula tarantula), spined loach ( Cobitis taenia), the common minnow (Phoxinus phoxinus), the common roach (Rutilus rutilus), the european bullhead (Cottus gobio), and the three-spined stickleback (Gasterosteus aculeatus).

There also be a range of other wildlife such as swans, ducks, voles and myriad types of water insects.