It continues south over the Western Ring Road through Glenroy, under CityLink and over the Craigieburn railway line through Strathmore, until the intersection with Fletcher Street in Essendon, where it shares surface tram tracks.

It continues south to eventually terminate at Moonee Ponds Junction, where it meets Mount Alexander Road and Ascot Vale Road in Moonee Ponds.

Tram route 59 passes along the length of Pascoe Vale Road between Fletcher Street in Essendon and Moonee Ponds Junction.

[6] Pascoe Vale Road was signed as Metropolitan Route 35 between Broadmeadows North and Moonee Ponds in 1965, heading east along Barry Road to eventually meet Hume Highway in Campbellfield.

Metropolitan Route 35 continues south along Ascot Vale Road eventually to Laverton.