The route heads through wooded areas and passes through the residential community of Turkey City.

PA 338 crosses over I-80 again and runs near more farmland before turning southeast through a woodland area.

PA 338 heads north-northeast through a mix of farmland and woodland with some residences, crossing into the borough of Knox.

At this point, the route becomes Main Street and runs past homes, intersecting PA 208.

PA 338 enters Ashland Township and continues northwest through more rural areas, turning to the north before ending at US 322 in Kossuth.