The northern terminus is at a fork from PA 155 in Keating Township.

PA 607 begins at an intersection with PA 872 in the borough of Austin in Potter County, heading west on two-lane undivided Main Street.

The route heads into forested areas of hills and leaves Austin for Portage Township, where it becomes an unnamed road.

PA 607 enters Keating Township and continues northwest through rural land, passing through tracts of Susquehannock State Forest.

Farther northwest, the road makes a curve to the west before a sharp turn to the south.