Its northern terminus is at U.S. Route 6 (US 6) in Blooming Grove, running south to US 209 in Delaware Township.

PA 739's southern end is at the Dingman's Ferry Bridge at the Delaware River near Layton, New Jersey.

Traveling northward from the southern terminus, the highway passes through the Delaware Water Gap National Recreation Area.

Soon after the intersection with Sproul Road, PA 739 leaves the Delaware Water Gap National Recreation Area.

[7] The southernmost portion of PA 739 south of US 209, SR 2019, first appeared on the PennDOT's maps of Pike County in 1993.

[9] In 2003, the Pennsylvania Department of Transportation ordered excavation of the intersection of PA 739 and SR 2001 (Milford Road).