While surveying the Mason–Dixon line in 1767, the English astronomer Charles Mason and a team of colonial surveyors ascended Chestnut Ridge and passed within three miles of the southern tip of Quebec Run.

The whole is a deep melancholy appearance out of nature.”[6][7][8] At this time Quebec Run was blanketed by old-growth forest dominated by American chestnut, eastern hemlock, oak, and maple.

[9] A local legend exists about a group of Confederate soldiers who buried a large cache of gold, allegedly stolen from nearby Union banks during the Civil War, just north of the stream known as Quebec Run.

Mountain laurel and northern maidenhair fern are common along the higher slopes and ridges, while wood nettle and rhododendron thickets line the banks of many streams.

Wildlife includes white-tailed deer, beaver, skunk, wild turkey, red fox, coyote, timber rattlesnake, bobcat, and American black bear.