They catalyze the following general reactions: These membrane-associated enzymes have attracted great interest for the detoxification of organohalide pollutants.
Organohalide pollution is a serious global environmental issue affecting soil and groundwater; and reductive dehalogenases offer a promising natural tool for bioremediation.
[1] The examples are those that can dechlorinate chloroform (TmrA), PCE (PceA), TCE (TceA), and VC (VcrA).
[10][11] Ultracentrifugation, membrane solubilization and a series of liquid chromatography are the commonly employed techniques to the isolation and purification.
The first membrane-associated respiratory reductive dehalogenase was heterologously expressed in a soluble and active form and purified using Bacillus megaterium.