[6] The nuclear protein binds to a purine-rich sequence known as the PU-box found on enhancers of target genes, and regulates their expression in coordination with other transcription factors and cofactors.

Multiple transcript variants encoding different isoforms have been found for this gene.

The majority of the cells expressing PU.1 in fibrotic conditions remain to be fibroblasts with a few infiltrating lymphocytes.

[10] The ETS domain is the DNA-binding module of PU.1 and other ETS-family transcription factors.

SPI1 has been shown to interact with: This article on a gene on human chromosome 11 is a stub.