Sainte-Marie (French pronunciation: [sɛ̃t maʁi] ⓘ) is a city in the province of Quebec, Canada.

It is the seat of La Nouvelle-Beauce Regional County Municipality, in Chaudière-Appalaches, Quebec, Canada.

It is located 59 kilometres (37 mi) south-east of Quebec City, on the Chaudière River.

The territory of Sainte-Marie was divided on several occasions, as population increased, in order to found neighbouring communities: Saint-Bernard, Saint-Isidore, Saint-Maxime-de-Scott (now Scott), Sainte-Marguerite, Sainte-Hénédine, Saint-Sylvestre, Saint-Elzéar, Saint-Séverin, Saints-Anges, and Vallée-Jonction.

In 1958, the village was constituted as a city and in 1959, Sainte-Marie-de-la-Nouvelle-Beauce was renamed as Sainte-Marie.