The second step involves cleavage of the cohesin subunit SCC1 (RAD21) by separase, which initiates the final separation of sister chromatids.
Esp1 is a separase protein that cleaves the cohesin subunit Scc1 (RAD21), allowing sister chromatids to separate at the onset of anaphase during mitosis.
Separase initiates the activation of Cdc14 in early anaphase[13] and Cdc14 has been found to dephosphorylate securin, thereby increasing its efficiency as a substrate for degradation.
The presence of this positive feedback loop offers a potential mechanism for giving anaphase a more switch-like behavior.
[12] This article incorporates text from the United States National Library of Medicine, which is in the public domain.