Single cell epigenomics

[9] It is a technique used in molecular biology to identify accessible DNA regions, equivalent to DNase I hypersensitive sites.

[9] ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.

[9] Single-cell ChIP-seq is extremely challenging due to background noise caused by nonspecific antibody pull-down,[1] and only one study so far has performed it successfully.

This study used a droplet-based microfluidics approach, and the low coverage required thousands of cells to be sequenced in order to assess cellular heterogeneity.

[17][18] Additionally, advances have been made in the analysis of Hi-C data,  allowing for the enhancement of HiC datasets to generate even more accurate and detailed contact maps and 3D models.

An overview of methods for single-cell epigenomic sequencing. Each method is labelled on the bottom row. Arrows are coloured by method, showing the flow from starting material to sequence data. Adapted from [ 1 ]
One method for single cell DNA methylation sequencing [ 4 ]
Comparison of single cell DNA methylation sequencing methods in terms of coverage as at 2015 on Mus musculus
Two methods for single-cell ATAC-seq [ 8 ]