Sortase B

This is believed to result in a different orientation of the protein on the surface of the cell, potentially affecting substrate access.

[6] The sortase B enzyme catalyzes a cell wall sorting reaction with a surface protein where a signal NXTN motif is cleaved.

The amino groups of the pentaglycine cross-bridges linked to the lipid II peptidoglycan precursor molecules are thought to function as nucleophile resolving acyl intermediates and creating an amide bond between the surface protein and lipid II with subsequent incorporation of this intermediate into the cell wall envelope.

IsDC remains buried within the cell wall, not surface located like IsDA and IsDB anchored by Sortase A.

[12] SrtB, in particular, has gained much attention and is recognized as a promising target[13] and deletion of its gene in gram-positive bacteria will lead to serious virulence defects.