TaqMan

[5] The quencher molecule quenches the fluorescence emitted by the fluorophore when excited by the cycler’s light source via Förster resonance energy transfer (FRET).

TaqMan probes are designed such that they anneal within a DNA region amplified by a specific set of primers.

TaqMan probes can be conjugated to a minor groove binder (MGB) moiety, dihydrocyclopyrroloindole tripeptide (DPI3), in order to increase its binding affinity to the target sequence; MGB-conjugated probes have a higher melting temperature (Tm) due to increased stabilization of van der Waals forces.

TaqMan probe-based assays are widely used in quantitative PCR in research and medical laboratories: 1.

TaqMan RT-PCR resources- primer databases, software, protocols Archived 2010-11-25 at the Wayback Machine 2.