Tissue culture
The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows.
In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture.
In 1913, E. Steinhardt, C. Israeli, and R. A. Lambert grew vaccinia virus in fragments of guinea pig corneal tissue.
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine.
"[5][6][7] In modern usage, "Tissue culture" generally refers to the growth of cells from a multicellular organism in vitro.
Tissue culture is an important tool for the study of the biology of cells from multicellular organisms.
Eric Simon, in a 1988 NIH SBIR grant report, showed that electrospinning could be used to produce nano- and submicron-scale polymeric fibrous scaffolds specifically intended for use as in vitro cell and tissue substrates.
[10] The technique of plant tissue culture, i.e., culturing plant cells or tissues in artificial medium supplemented with required nutrients, has many applications in efficient clonal propagation (true to the type or similar) which may be difficult via conventional breeding methods.
In addition, fruit plants or flowers can be obtained free of viruses, phytoplasmas, viroids.
The second method is primary explant culture, in which fragments derived from animal tissue are attached to a surface using an extracellular matrix component (ECM), such as collagen or a plasma clot.
