Transcription factor II E (TFIIE) is one of several general transcription factors that make up the RNA polymerase II preinitiation complex.
[1] It is a tetramer of two alpha and two beta chains and interacts with TAF6/TAFII80, ATF7IP, and varicella-zoster virus IE63 protein.
[2] TFIIE recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH.
Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
[3][4][5][6] TFIIE is thought to be involved in DNA melting at the promoter: it contains a zinc ribbon motif that can bind single stranded DNA.