Good first prepared tricine to buffer chloroplast reactions.
Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets.
This allows for low molecular weight proteins to be separated in lower percent acrylamide gels.
Tricine has been documented in the separation of proteins in the range of 1 to 100 kDa by electrophoresis.
[3] Tricine has also been found to be an effective scavenger of hydroxyl radicals in a study of radiation-induced membrane damage.