Viability assay

[3] Viability assays provide a more precise basis for measurement of an organism's level of vitality.

As with many kinds of viability assays, quantitative measures of physiological function do not indicate whether damage repair and recovery is possible.

[12] An assay of the ability of a cell line to adhere and divide may be more indicative of incipient damage than membrane integrity.

Some of its limitations include that it does not account for total viability and it is not particularly sensitive to low-viability assays; however, it is known for its quick pace.

The "tadpoling" method can be used to measure culture viability accurately, which is what depicts its main separation from "frogging".

This plated viability assay measures various yeast viability though a method called "frogging". The research is completed through drop-inoculation techniques. Research has since been conducted on "tadpoling", which is a variation of "frogging" that is completed by keeping the test cells diluted in liquid throughout their examination. [ 1 ]
Flow cytometry using 7-Aminoactinomycin D (7-AAD), wherein a lower signal indicates viable cells. Therefore, this case shows good viability (viability of the cells in flow cytometry should be around 95% but not less than 90%. [ 8 ] ).