[2] Wolfson Children's Hospital also features the only Florida Department of Health-designated pediatric trauma referral center in Jacksonville, Florida, and the only American College of Surgeons-verified, Level 1 pediatric trauma center in the region.

[3][4] The hospital is situated on the St. Johns River waterfront in Downtown Jacksonville and is part of a large medical complex which also includes Baptist Medical Center Jacksonville, Baptist MD Anderson Cancer Center, and Nemours Children's Clinic.

[6] The hospital grew incrementally during its early years, establishing a Cystic Fibrosis Center in 1963, a Children's Tumor Program in 1970 and an eight-bed Neonatal Intensive Care Unit in 1971.

[11] Ellen Cavert was elected chairman of the Women's Board of Jacksonville Children's Hospital with a mission to raise funds and awareness.

In December 1977, the group held its inaugural Christmas antiques show to raise funds for the expansion of cancer treatment for children.

[15] The partnership between Baptist and Nemours will consolidate some specialized children's medical services and transfer others, like the cystic fibrosis and tumor programs.

[16] A nine-bed pediatric cancer unit opened in October 1989 for children with chronic health problems.

[18] The new consortium was designed to eliminate the duplication of medical services and provide comprehensive pediatric care, research, and education in the region.

[18] The inaugural season of the Florida Forum, a new fund-raising project of the Women's Board of Jacksonville Wolfson Children's Hospital, kicks off in 1992.

[20] In 1993, a new Wolfson Children's Hospital opened, offering a range of inpatient and outpatient services, including a level III Neonatal Intensive Care Unit, Pediatric Intensive Care Unit, Children's Emergency Center, and specialized programs such as pediatric oncology, radiology, pathology and research.

Michael D. Aubin, the founding administrator and CEO of St. Joseph's Children's Hospital in Tampa, Florida, succeeds Freeman on Jan. 1, 2011.

[23] In 2012, Wolfson Children's Hospital expanded with the opening of the new J. Wayne and Delores Barr Weaver Tower on the Baptist Medical Center Jacksonville campus.

[25] The first of what is now five satellite Wolfson Children's Emergency Centers opened in May 2014, at Baptist Clay Medical Campus.

[28] The Kids Kare Critical Care and Trauma Transport Program unveiled a new helicopter, a Bell 407, in November 2020.

Wolfson Children's Hospital achieved Magnet status by the American Nurses Credentialing Center in 2007 and again in 2012 and 2017, an international recognition of excellence in patient care.