The 1999–2000 Tennessee Lady Volunteers basketball team represented the University of Tennessee as a member of the Southeastern Conference during the 1999–2000 women's college basketball season.

Coached by Pat Summitt, the Lady Volunteers finished 33–4 (13–1 SEC).

Riding a 20-game winning steak entering the National Championship game, that included a win over No.

1 UConn, they ended the season runner-up after losing to the Huskies, 71–52.

[1] Roster [2] [7]