The 2010 U.S. Open Cup tournament proper features teams from the top five levels of the American Soccer Pyramid.
Note: Scorelines use the standard U.S. convention of placing the home team on the right-hand side of box scores.
All 15 eligible teams will automatically qualify rather than 8 from each league as in years past, leaving an additional slot to be filled .
The Abbotsford Mariners, Ottawa Fury, Toronto Lynx, Thunder Bay Chill, Vancouver Whitecaps Residency, Victoria Highlanders and Forest City London are not eligible because they are Canadian clubs.
Though it was originally scheduled to feature nine clubs organized into three groups of three followed by an elimination bracket, a lightning storm canceled the first day of games and Brazil Soccer Academy was disqualified for failing to show.
On May 5, 2010 the USSF announced that the remaining slot would be allotted to the winner of a game between the Sonoma County Sol and PSA Los Gatos Storm, two California-based clubs in the US Soccer amateur structure.