The top four overall Major League Soccer teams from the 2009 season not already qualified for the 2010–11 CONCACAF Champions League earned qualification[1] as well as four clubs from the Primera División de México.

The teams involved were selected based on qualification rules set by their respective leagues.

On April 28, 2010, MLS announced that for the 2010 season the top four teams from the 2009 MLS regular season standings not competing in the 2010–11 CONCACAF Champions League would qualify for SuperLiga 2010.

The Primera División de México announced its participants would be the top four team from the 2009 overall standings (Clausura 2009 and Apertura 2009) that were not competing in the 2010–11 CONCACAF Champions League.

The SuperLiga 2010 participants are as follows: From Major League Soccer: From Primera División de México: There were two groups of four teams.