Lyngby Boldklub and AC Horsens finished the 2020–21 season of the Superliga in 11th and 12th place, respectively, and were relegated to the 1st Division.

They replaced Viborg FF and Silkeborg IF, who were promoted to the 2021–22 Danish Superliga.

Note: Flags indicate national team as has been defined under FIFA eligibility rules.

The top 6 teams will compete for 2 spots in the 2022–23 Danish Superliga.

The bottom 6 teams will compete to avoid the 2 relegations spots to the 2022–23 Danish 2nd Division.