31 teams were awarded automatic bids as champions of their conferences after the Ivy League opted out of the 2021 softball season.
The tournament culminated with eight teams playing in the 2021 Women's College World Series at USA Softball Hall of Fame Stadium in Oklahoma City.
16 National Seeds were announced on the Selection Show, on Sunday, May 16 at 9 p.m. EDT on ESPN2.
Teams in bold advanced to the Women's College World Series.
† = From NCAA Division I Softball Championship Results The following players were members of the Women's College World Series All-Tournament Team.
The columns RF, SR, WS, NS, F, and NC respectively stand for the Regional Finals, Super Regionals, College World Series Teams, National Semi-Finals, Finals, and National Champion.
For the first time ever Westwood One will provide nationwide radio coverage of every game in the Women's College World Series.
Ryan Radtke and Leah Amico return as the broadcast team.
For just the fourth time in the history of the women's softball tournament, ESPN covered every regional.
ABC will air a super regional game for the first time in tournament history.