After sustaining heavy casualties at the battles of Shiloh and Perryville, it was amalgamated with the 1st Tennessee Infantry Regiment.

The regiment was organized on September 10, 1861, at Camp Trenton, from new and existing companies of volunteer infantry.

Its 833 men were from the counties of Benton, McNairy, Obion, Henderson, Decatur, Crockett, Weakley, and Carroll.

[1] The regiment participated in many conflicts from Murfreesboro to Atlanta, endured Hood's winter operations, and ended the war in North Carolina attached to Palmer's Brigade.

The 1st and 27th Tennessee had 83 casualties of the 457 at Murfreesboro, reported 14 killed and 75 wounded at Chickamauga, and in late 1863, totaled 456 men and 290 arms.