[3] ] 6-pyruvoyltetrahydropterin synthase (PTPS) can be found in the cytoplasm as well as the nucleus of cells according to immunohistochemical studies conducted.

It has also been found that in higher species 6-pyruvoyltetrahydropterin synthase (PTPS) can undergo post-translational modification.

A 12-stranded antiparallel b-barrel is formed by the trimer of dimers and creates a pore within PTPS, with a 6 to 12 A ̊ diameter.

[8] Tetrahydrobiopterin deficiency leads to hyperphenylalaninemia and the inability to make neurotransmitters such as dopamine and serotonin.

[10] PTPS deficiency has been shown to lead to severe mental retardation, delayed motor development, and seizures.