The Allegory of Virtue and Vice is an oil-on-panel painting by the Italian High Renaissance painter Lorenzo Lotto, dating to 1505.

The painting originally formed the protective cover of the Portrait of Bernardo de' Rossi, the bishop of Treviso who was Lotto's patron at the time.

When it was opened to display the portrait, the inscription (on the reverse of the Allegory) would have been revealed: The painting was brought to Parma when de' Rossi fled Treviso, and there it became part of the Farnese collection, from which it was bought in 1803; after a series of different possessors, it arrived in the United States in 1935, and was donated to the current museum four years later.

The painting is an allegoric scene with the bishop's coat of arms lying on a tree in the center of the composition.

The theme is perhaps derived from an engraving by Albrecht Dürer, which also includes a similar tree with moral symbols.