The Portrait of Bishop Bernardo de' Rossi is an oil-on-panel painting by the Italian High Renaissance painter Lorenzo Lotto, dating to 1505.

The work dates to Lotto's stay in Treviso, and featured a cover with title, signature and dates, identified as the Allegory of Virtues and Vices now at the National Gallery of Art in Washington, United States.

The portrait was brought to Parma by Bernardo de' Rossi when he fled there in 1524.

Such an attention to details was inspired by Antonello da Messina, in turn influenced by the Flemish art and perhaps directly by Northern European artists such as Albrecht Dürer, whose drawings could have been seen by Lotto The red mantel is backed by a green embroidered drapery, a common element of Venetian painting of the period.

The roll is perhaps an allusion to the sentence against the conjurers who had attempted to de Rossi's life two years before.