DNA barcoding is an alternative method to the traditional morphological taxonomic classification, and has frequently been used to identify species of aquatic macroinvertebrates (generally considered those large enough to be seen without magnification).
Accurate diagnosis depends on low intraspecific variation compared with that between species, a short DNA sequence such as Cytochrome Subunit Oxidase I gene (COI), would allow precise allocation of an individual to a taxon.
Moreover, sorting invertebrates into different size categories is useful, since specimens in a sample can vary widely in biomass, depending on species and life stage.
Others, validate the use of next-generation sequencing (NGS) technologies in environmental samples to evaluate water quality in marine ecosystems[9] and in freshwater biodiversity studies,[10] including macroinvertebrate species assessment.
[11] Most of the recent studies are based on advancing eDNA approaches' implementation, field validation, platform and barcode choice or database limitations.