SR 389 stretches from the Utah border at Colorado City, southeast to Pipe Spring National Monument, and ends at U.S. Route 89A in Fredonia; it is the only major east–west route between these two towns, and also serves to connect Fredonia with points farther west such as St. George, Utah.

The highway takes a more easterly course as it enters the Kaibab Indian Reservation.

Within the reservation, the highway passes near the Pipe Spring National Monument.

Once it passes the monument, the highway begins to head towards the northeast towards Fredonia.

It leaves the Kaibab Indian Reservation and enters the city of Fredonia before reaching its eastern terminus at US 89A.