Barkers Creek is a tributary of the Guyandotte River, 8 miles (13 km) long,[3] in southern West Virginia in the United States.

Via the Guyandotte and Ohio rivers, it is part of the watershed of the Mississippi River, draining an area of 36.85 square miles (95.4 km2) in a rural area on the unglaciated portion of the Allegheny Plateau.

[4] Barkers Creek's entire course and drainage area are in southeastern Wyoming County.

It rises in the eastern extremity of Wyoming County, near the common boundary of Wyoming, Raleigh, and Mercer counties, approximately 3 miles (4.8 km) northeast of Arista, and flows initially westward, then turns northwestward through the unincorporated communities of Bud, Alpoca, and Tralee.

In its lower course the creek is paralleled by West Virginia Route 10.