Bis-tris methane

Bis-tris methane, also known as BIS-TRIS or BTM, is a buffering agent used in biochemistry.

Bis-tris methane is an organic tertiary amine with labile protons having a pKa of 6.46 at 25 °C.

It is an effective buffer between the pH 5.8 and 7.2.

Bis-tris methane binds strongly to Cu and Pb ions as well as, weakly, to Mg, Ca, Mn, Co, Ni, Zn and Cd.

[2] This article about an amine is a stub.

Skeletal formula of bis-tris methane
Skeletal formula of bis-tris methane
NFPA 704 four-colored diamond Health 2: Intense or continued but not chronic exposure could cause temporary incapacitation or possible residual injury. E.g. chloroform Flammability 0: Will not burn. E.g. water Instability 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g. liquid nitrogen Special hazards (white): no code