The assumptions [3] used for estimating the number of potential pharmacologically active molecules, however, use the Lipinski rules, in particular the molecular weight limit of 500.
The estimate also restricts the chemical elements used to be Carbon, Hydrogen, Oxygen, Nitrogen and Sulfur.
This number is often misquoted in subsequent publications to be the estimated size of the whole organic chemistry space,[4] which would be much larger if including the halogens and other elements.
As of October 2024, 219 million molecules were assigned with a Chemical Abstracts Service (CAS) Registry Number.
[12][13] Generation of chemical spaces may involve creating stoichiometric combinations of electrons and atomic nuclei to yield all possible topology isomers for the given construction principles.