Most recently Company C has served in Bosnia, at Guantanamo Bay, Cuba, New Orleans following Hurricane Katrina, Iraq and Afghanistan.

In 1861 mobilized for the Civil War as the Cambridge City Guard with the 20th Massachusetts Volunteer Infantry (The Harvard Regiment).

Among the officers of the company were Major Paul J. Revere, and Lieutenant Oliver Wendell Holmes Jr.

They received standard infantry training so that they could defend themselves, but there are very few documented instances of any pioneer troops unslinging their rifles.

The year of training ended in December 1941 but the company's service continued after the Japanese attack on Pearl Harbor brought the United States into the Second World War.

Company H, 101st Infantry deployed to Europe with the Yankee Division and fought from Normandy across Germany to meet the Soviets in Czechoslovakia at war's end.

In September 2005, Company C mobilized as an element of JTF Yankee for rescue and security operations in New Orleans following Hurricane Katrina.

In August 2010, Company C 1-181 IN deployed for one year of service with the International Security Force in Afghanistan in support of Operation Enduring Freedom.

The company provided command and control at Camp Alamo in Kabul and conducted security operations in Khost, Paktya and Paktika Provinces.