[2] Czech Republic returned home from Rio de Janeiro with a total of 10 medals (1 gold, 2 silver, and 7 bronze), marking the country's second-most successful Olympics, behind tallies of 11 achieved at Atlanta 1996 and London 2012.

[6] Despite witnessing her three-peat bid come to an end with a bronze at the Games, double Olympic champion Barbora Špotáková cemented her place in history as the first woman to score three medals in the javelin throw.

[7] Mountain biker Jaroslav Kulhavý closed out the nation's Olympic campaign by taking home the silver medal in the men's cross-country race, losing his title defense to Switzerland's Nino Schurter.

Thirteen of the nation's past Olympic medalists returned, including four defending champions from London 2012: javelin thrower Barbora Špotáková, modern pentathlete David Svoboda, single sculls rower Miroslava Knapková, and mountain biker Jaroslav Kulhavý.

[2] 2008 trap shooting champion David Kostelecký, synchronized swimmer Soňa Bernardová, and former cross-country skier Kateřina Nash in mountain biking headed the full roster of Czech athletes by participating in their fifth Olympics as the most experienced competitors.

[1][9] Rifle shooting rookie Filip Nepejchal and freestyle swimming teen Barbora Seemanová, both of whom competing under 18, were Czech Republic's youngest competitors, with Kostelecký rounding out the field as the oldest member (aged 41).

[16] Meanwhile, one additional boat was awarded to the Czech squad in men's C-2 1000 m by virtue of a top two national finish at the 2016 European Qualification Regatta in Duisburg, Germany.

The sprint canoeing team, led by London 2012 bronze medalist Josef Dostál in the men's kayak four, was named to the Olympic roster on 7 June 2016.

Due to the lack of eligible NOCs for Africa on the list, the unused berth was added to the Czech mountain biking team as the next highest-ranked nation, not yet qualified, in the women's cross-country race.

2010 Youth Olympian Alexander Choupenitch secured a spot in the men's foil as one of the two highest-ranked fencers coming from the European zone in the FIE Adjusted Official Rankings.

[26] Meanwhile, Barbora Kodedová granted an invitation from UIPM to compete in the women's event, as one of the next highest-ranked eligible modern pentathletes, not yet qualified, in the World Rankings as of 1 June 2016.

London 2012 Olympian Iveta Vacenovská was automatically selected among the top 22 eligible players in the women's singles based on the ITTF Olympic Rankings.

[34] Meanwhile, Lubomír Jančařík, Dmitrij Prokopcov, and Hana Matelová granted their invitations from ITTF to compete in each of their respective singles events as one of the next seven highest-ranked eligible players, not yet qualified, on the Olympic Ranking List.

Two-time Olympian Vendula Frintová was ranked among the top 40 eligible triathletes in the women's event based on the ITU Olympic Qualification List as of 15 May 2016.

[39] The Czech Republic women's beach volleyball team qualified directly for the Olympics by virtue of their top two national finish at the 2016 FIVB Continental Cup in Sochi, Russia.

The Czech Republic has received a spare host berth freed up by Brazil as the next highest-ranked eligible nation, not yet qualified, to send a wrestler competing in the women's freestyle 63 kg to the Olympics, based on the results from the World Championships.