Hillsborough Avenue is a major east–west arterial road in Hillsborough County, Florida, also designated as State Road 580, U.S. Highway 41 or U.S. Highway 92 in various places.

This route takes it through the city of Tampa, where it crosses the Hillsborough River.

Two blocks east of the interchange with Interstate 275, the road encounters the intersection with Nebraska Avenue, where an overlap with US 41 begins.

Along the way, the overlap between US 41 and 92 encounters a railroad crossing with the CSX Clearwater Subdivision.

The US 41-92 overlap ends at North 40th Street where southbound US 41 turns right, and eastbound US 92 continues along Hillsborough Avenue The street runs along a bridge over the CSX Neve Spur then has an intersection with Florida State Road 583.