[1] G. thermoglucosidasius is classified as a thermophile as optimal growth occurs at 60 °C (140 °F), although strains have demonstrated ability to grow at temperatures between 37 °C (98.6 °F) and 68 °C (154.4 °F).

[2] Under a microscope, the cells are observed to occur either singly or in short chains, while possessing peritrichous fagella for motility or appearing non-motile.

[2] Vegetative G. thermoglucosidasius sporulates, producing one endospore per cell located terminally or subterminally in slightly swollen or non-swollen sporangia.

G. thermoglucosidasius uses mixed-acid fermentation in anaerobic conditions, producing lactate, succinate, formate, ethanol, acetate and carbon dioxide.

Most thermoglucosidasius strains have hydrolytic activity to starch, gelatin, and pullulan, as well as producing acid from adonitol, cellobiose, inositol, and D-xylitol.