It travels through Lowndes, Lanier, Berrien, Tift, Irwin, and Ben Hill counties connecting the Valdosta and Fitzgerald metropolitan areas.

The route runs north-northeast, past Freedom Park and passes through Moody Air Force Base and intersects SR 122 at the meeting point of Lowndes, Berrien, and Lanier counties.

In the central part of town, the four concurrent routes intersect SR 168 (East McPherson Avenue).

Farther into town, SR 125 splits off to the northeast and enters Irwin County.

They run concurrent to the northeast, through Irwinville, and to the east-southeast until SR 32 (Mystic Highway) splits off to the southeast.