The route begins at an intersection with US 129/SR 11/SR 76/SR 125 in Nashville.

It heads southeast to an intersection with SR 64/SR 135 north of Lakeland, where SR 64 begins a concurrency to the east.

Farther to the east, SR 64 leaves the concurrency to the northeast.

Northwest of its eastern terminus is an intersection with US 221/SR 31.

The highway continues to the southeast until it meets its eastern terminus, an intersection with SR 37 west of Homerville.