It travels from Interstate 75 (I-75) in Ashburn to U.S. Route 221 (US 221) in rural Jeff Davis County.

It then travels to the east toward Fitzgerald, concurrent with SR 112 for approximately 3 miles (4.8 km) before departing.

The highway then goes through rural Turner and Irwin counties before an intersection with SR 125 just west of Fitzgerald.

It shares a concurrency with US 319 as the highway leaves Fitzgerald and goes through rural Ben Hill County.

SR 107 shares a brief concurrency with US 441 before turning east into rural Coffee and Jeff Davis counties.