It connects Thebes with Karystos on southern Euboea, via Chalcis and Eretria.

It crosses the A1 motorway east of Thebes, and runs north to Chalcis.

At Chalcis it crosses the Euripus Strait, and enters the island of Euboea.

The GR-44 continues to the southeastern part of the island, passing along the south coast through the towns Eretria, Amarynthos and Aliveri.

Beyond Aliveri it leaves the coast, and it runs south through Styra, ending in Karystos.