High-performance thin-layer chromatography

This mode achieves separation by allowing the mobile phase to enter the plate layer precisely along an outer circular path, after which it flows toward the center at a nearly constant speed.

This approach maximizes sample capacity while minimizing time, layer, and mobile phase consumption, making it the most cost-effective HPTLC technique.

After the selection of the stationary phase, plates are generally washed with methanol and dried in an oven to remove excess solvent.

[5][6] Notable HPTLC devices such as the Linomat 5 and the Automatic TLC Sampler 4 (ATS 4) by CAMAG function very similarly by having the automated 'spray-on' sample application technique.

[4][5] This automated 'spray-on' technique is useful to overcome the uncertainty in droplet size and position when the sample is applied to the TLC plate by hand.

Additionally, automation provides high resolution and narrow bands since the solvent evaporates immediately as the sample makes contact with the plate.

[7] Alternatively, the Nanomat 4 and ATS 4 by CAMAG are manually operated where the sample is applied via spot application using a capillary pipette.

[4][5] Upon chromatographic detection, HPTLC plates are usually developed in saturated twin-trough chambers with filter paper for optimal outcomes.