Hot cells are used to inspect spent nuclear fuel rods and to work with other items which are high-energy gamma ray emitters.
Hot cells are of nuclear proliferation concern, as they can be used to carry out the chemical steps used to extract plutonium (whether weapons grade or not) from reactor fuel.
Hot cells are commonly used in the nuclear medicines industry: The user must never be subject to shine paths that are emitted from the radioactive isotopes and therefore there generally is heavy shielding around the containment boxes, which can be made out of 316 stainless steel or other materials such as PVC or Corian.
The amount of radioactivity present in the hot cell, the energy of the gamma photons emitted by the radioisotopes, and the number of neutrons that are formed by the material will prescribe how thick the shielding must be.
Also if some actinide materials such as californium or spent nuclear fuel are used within the hot cell then a layer of water or polyethylene may be needed to lower the neutron dose rate.
Lead loaded gloves are often used in conjunction with tongs as they offer better dexterity and can be used in low radiation environments (such as hot cells used in hospital nuclear medicine labs).