Plasmid DNA containing the gene, and intended for intracellular delivery, is attached to the nanostructure surface.
As one of the types of transfection, the term is derived from two words – impalement and infection.
One of the features of impalefection is spatially resolved gene delivery that holds potential for such tissue engineering approaches in wound healing as gene activated matrix technology.
[2] Though impalefection is an efficient approach in vitro, it has not yet been effectively used in vivo on live organisms and tissues.
[3] Vertically aligned carbon nanofiber arrays prepared by photolithography and plasma enhanced chemical vapor deposition are one of the suitable types of material.