CRACD-like protein

The CRACD-L protein is proline rich and asparagine, isoleucine, phenylalanine, and tyrosine poor.

[12] The CRACD-L protein has one domain called the DUF4592 motif and spans amino acids 131–239.

The CRACD-L protein predicted secondary structure is composed of 50% alpha helixes, 8.9% beta sheets, and 17.9% turns.

[7] The orthologs, including the elephant shark, horse, rock dove, and chimp, are also predicted to be located in the nucleus.

[19] The promoter region of CRACDL is approximately 1340 base pairs with various predicted transcription factors.

[20] The glial cells missing homolog 1 and the oligodendrocyte lineage transcription factors are notable because CRACDL is highly expressed in the brain.

[23] The proteins it phosphorylates are the principle components of neurofibrillary tangles in Alzheimer disease.

[5][6][8][9] The interaction between GSK3B and CRACDL was predicted using anti bait coimmunoprecipitation, pull down, tandem affinity purification, fluorescence polarization spectroscopy, protein kinases assay, two hybrid, and confocal microscopy experiments.

[27] The KIAA1211 has an ortholog in the bacteria Proteus vulgarism, indicating the paralog duplicated 4290 million years ago, before KIAA1211L.

The CRACDL gene is similar and conserved in mammals, birds, reptiles, amphibians, and fish.

Conceptual Translation CRACDL Part 1. The upstream stop codon is orange, the exon boundaries are highlighted blue, the start codon is green, the predicted nuclear location signal is denoted by a purple box, the conserved regions are highlighted orange, the phosphorylation sites are highlighted yellow, the predicted SUMOylation sites are denoted by green boxes, the DUF4592 Motif is highlighted red.
Conceptual Translation CRACDL Part 2. The exon boundaries are highlighted blue, the conserved regions are highlighted orange, the predicted SUMOylation sites are denoted by green boxes, the stop codon is red, the predicted miRNA 132 target is underlined in purple, and the polyadenylation signal is highlighted magenta.
CRACDL Schematic. This figure is a schematic illustration of CRACDL. The nuclear location signal is blue, the phosphorylation sites are red, the SUMOylation sites are grey, and the DUF4592 motif is orange.